Antibodies products online

2,3-dinor-6-keto-PGF1 alpha Affinity Resin**

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:01:39 -0600

Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1 alpha and 2,3-dinor-6-keto-PGF1 alpha. 6-keto-PGF1 alpha is produced by thenon-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1 alpha.

3T3 Cell Lysate

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:02:23 -0600

The 3T3 cell line is a spontaneously immortalized mouse fibroblast cell line established from Swiss mouse embryonic tissue.

5X Extraction Reagent

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:07:20 -0600

Applications
Concentrated lysis buffer for preparation of cell and tissue extracts

Description
Tris buffer containing detergents

For research use only, not for diagnostic or therapeutic use.

6-keto-PGF1 alpha Affinity Resin **

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:07:46 -0600

Apoptosis & Cancer

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:19:14 -0600

The major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid [13(S)-HODE] via lipoxygenase or [13(R)-HODE] by p450 enzymes. Alternatively, cyclooxygenase metabolism of linoleic acid results in the production of hydroxyeicosatetraenoic acid [15(S)-HETE]. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to mediate cell adhesion and excessive proliferation that is critical in the formation of tumors and metastases, as well as the formation of atherogenic plaques.

14-3-3 beta Monoclonal Antibody (4E1)

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:19:51 -0600

14-3-3 proteins are highly conserved proteins that play a role in both signal transduction and progression through the cell cycle by binding to and regulating a number of different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms.

Acinus (CT) Polyclonal Antibody

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:20:12 -0600

Acinus (apoptotic chromatin condensation inducer in the nucleus) is a nuclear factor that induces apoptotic chromatin condensation after cleavage by caspase-3 without inducing DNA fragmentation. Acinus is ubiquitously expressed and three different spliced forms of acinus have been identified in human and mouse and are designated AcinusL, AcinusS, and AcinusS?.

Adiponectin Polyclonal Antibody

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:21:45 -0600

Adipose tissue is the source for a variety of bioactive molecules that regulate metabolism and immunity termed adipokines, most of which are elevated in the circulation of obese individuals. Adiponectin functions as an insulin sensitizing factor, resulting in reduced hepatic glucose production, increased glucose uptake in the muscle, and increased fatty acid oxidation in the liver and muscle. In contrast to most adipokines, adiponectin is detected at high levels in normal human plasma, but is often reduced in obese subjects and in those with increased insulin resistance and type 2 diabetes. Due to its suggested protective role in insulin resistance, adiponectin may be a useful pharmacological target for various metabolic diseases.

Akt Polyclonal Antibody

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:22:14 -0600

The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Arg8-Vasopressin EIA Kit

admin@catalogchinese.com (Hailiang Wu) — Mon, 25 Aug 2008 23:22:52 -0600

Arginine vasopressin (AVP) is a nonapeptide hormone produced in magnocellular neurons of the hypothalamus, which functions as an antidiuretic in the kidney and systemically as a regulator of vasoconstriction, blood volume, and blood pressure. AVP acts by binding to a set of specific seven transmembrane domain-containing G-protein coupled receptors (GPCR) classified into three groups: V1a (V1), V1b (V3), and V2. V1b receptors stimulate adrenocorticotropic hormone (ACTH) release in the anterior pituitary, but may also participate in corticotropin-releasing hormone (CRH) release by the hypothalamus. The V1b receptor signals through coupling of Gq/11 alpha subunits, activating phospholipase C and release of intracellular Ca2+ via phosphoinositide second messengers. V1b receptor overexpression is a common marker for ACTH-secreting corticotropic adenomas. V2 receptors in the collecting ducts of the kidneys stimulate adenylyl cyclase activity, regulating the reabsorption of water via aquaporin channels.

Human IgG1 Lambda

admin@catalogchinese.com (Hailiang Wu) — Tue, 26 Aug 2008 20:42:36 -0600

Product Name
Human IgG1 Lambda

Catalog Number
GTX76676

Product Description:
Human IgG1 Lambda Purified Myeloma Protein

Application Note:
Identity is determined by immunoelectrophoresis, Ouchterlony and indirect ELISA. Protein concentration is measured using E^0.1% = 1.4 at 280nm. T

Suggested Applications:
Immunoassay, Immunodiffusion, Immunoelectrophoresis. The usefulness of this product in other applications has not been determined.

Host:
Human

Isotype:
IgG1

Lightchain:
lambda

Form:
Liquid

Concentration:
1.1 mg/ml

Purity:
Fractionation/ion-exchange/affinity chromatography

Purification Note:
From human plasma

Storage Buffer:
20 mM TBS, pH 8.0. No preservatives have been added

Storage:
Aliquot and store at -20 °C or below. Avoid repeated freezing and thawing.

Direct References:
1.  McKinney, M., et al., J. Immunol. Methods, 96, 271   1987

2.  Bird, P., et al., J. Immunol. Methods, 71, 97   1984

3.  Tousch, D., et al., BioChromatography, 5, 30   1990

4.  Haaijman, J., et al., Immunology Today, 5, 56   1984

5.  Lew, A., J. Immunol. Methods, 72, 171   1984

6.  Steward, M., et al., J. Immunol. Methods, 78, 173   1985

7.  Kronick, M., et al., Clin. Chem., 29, 1582   1983

Rabbit Immunoglobulin IgG

admin@catalogchinese.com (Hailiang Wu) — Tue, 26 Aug 2008 20:42:52 -0600

Product Name
Rabbit Immunoglobulin IgG

Catalog Number
GTX76672

Product Description:
Rabbit Immunoglobulin IgG

Application Note:
For affinity column preparation use 3-5 mg/ml gel. For others usage use according to your protocol.

Background:
GeneTexs' immunoglobulin (IgG) / (Ig) preparations are used for affinity columns, blocking agents, and normal controls in a variety of immuno assays. These immuoglobulins are isolated and purified from pooled normal animal or human sera's by proprietary Protein A / Protein G/ Polyethylene Glycol or Ammonium Sulfate methods. Immunoglobulin are 95% to 97% pure as analyzed on SDS-page and are supplied in PBS with 0.05% sodium azide.

Clonality:
Polyclonal

Host:
Rabbit

Isotype:
IgG

Form:
Liquid

Concentration:
10 mg/ml

Purification Note:
From polyclonal serum

Storage Buffer:
Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage:
For long term storage, please keep at -70°C or below. Avoid repeared freeze-thaw cycles.

SOY Protein ELISA Kit.....Coming Soon

admin@catalogchinese.com (Hailiang Wu) — Tue, 26 Aug 2008 23:20:58 -0600

Each kit contains: anti-Soy sensitized 96-strip micro-well plate, 50ml sample buffer, 50 ml (20X) wash solution, enzyme-antibody conjugate (HRP anti-Soy), 12mL TMB substrate, 12mL stop solution and a Soy reference calibrator.

ICL's soy ELISA kit is for Research and Manufacturing Only; Not for use in diagnostic applications

Pig Haptoglobin ELISA Kit

admin@catalogchinese.com (Hailiang Wu) — Tue, 26 Aug 2008 23:21:01 -0600

Each kit contains: an anti-Pig Haptoglobin sensitized 96-strip micro-well plate, 50ml diluent, 50 ml(10X) wash solution, enzyme-antibody conjugate, 12mL TMB substrate, 12mL stop solution and pre-diluted reference standards.

ICL's Pig Haptoglobin ELISA Kit is For Research and Manufacturing Only; Not for Use in Diagnostic Applications

Bovine Casein ELISA Kit

admin@catalogchinese.com (Hailiang Wu) — Tue, 26 Aug 2008 23:21:13 -0600

Each kit contains: Rabbit anti-Bovine Casein sensitized 96-strip micro-well plate, 50ml diluent, 50 ml(10X) wash solution, enzyme-antibody conjugate (HRP Sheep anti-Bovine Casein), 12mL TMB substrate, 12mL stop solution and a bovine casein reference calibrator.

This kit will detect alpha, beta and kappa casein.

ICL's bovine casein ELISA kit is for Research and Manufacturing Only; Not for use in diagnostic applications

Affinity Purified Chicken anti Human IgG (absorbed Against Mouse. Goat and Rabbit)

admin@catalogchinese.com (Hailiang Wu) — Fri, 29 Aug 2008 23:36:58 -0600

Product Name
Affinity Purified Chicken anti Human IgG (absorbed Against Mouse. Goat and Rabbit)

Catalog Number
GTX77474

Product Description:
Affinity Purified Chicken anti Human IgG (absorbed Against Mouse. Goat and Rabbit)

Background:
The anti IgG shows a precipitin arc against human IgG in serum and against both the Fab and Fc fragments of papain digested IgG using immunoelectrophoresis. There is no reaction with mouse, goat or rabbit serum.. Chickens were immunized with highly purified normal pooled human IgG in Freund's adjuvant and eggs were collected. The IgY fraction was prepared and the antibody immunoaffinity purified. Anti human IgG was further absorbed against mouse, goat and rabbit IgG

Specificity:
Human

Cross Reactivity:
Human

Host:
Chicken

Species secondary against:
Human

Form:
Liquid

Storage Buffer:
0.01 M Phosphate Buffered Saline, 0.025% sodium azide

Storage Instruction:
4 °C

Affinity Purified Chicken anti-Human IgE

admin@catalogchinese.com (Hailiang Wu) — Fri, 29 Aug 2008 23:37:02 -0600

Product Name
Affinity Purified Chicken anti-Human IgE

Catalog Number
GTX77495

Product Description:
Affinity Purified Chicken anti-Human IgE

Background:
The anti IgE only reacts with IgE by ELISA, IEP and gel diffusion analysis. Serum known to be very low in IgE shows no reaction with the anti IgE. Chickens were immunized with a mixture of highly purified human IgE myeloma proteins in CFA. Serum and/or eggs were collected and serum or yolk derived IgG (IgY) fraction prepared. The resulting fluid was solid phase absorbed to make IgE epsilon chain specific and affinity purified off an antigen containing immunoabsorbant

Specificity:
Human

Cross Reactivity:
Human

Host:
Chicken

Species secondary against:
Human

Form:
Liquid

Storage Buffer:
PBS, pH 7.2, 0.1% sodium azide

Storage Instruction:
4 °C

Affinity Purified Chicken anti-Rabbit IgG h+l Absorbed Against Human and Mouse IgG [HRP]

admin@catalogchinese.com (Hailiang Wu) — Fri, 29 Aug 2008 23:39:37 -0600

Product Name
Affinity Purified Chicken anti-Rabbit IgG h+l Absorbed Against Human and Mouse IgG [HRP]

Catalog Number
GTX77914

Product Description:
Affinity Purified Chicken anti - Rabbit IgG h+l Absorbed Against Human and Mouse IgG (HRP-conjugated)

Application Note:
Western blot - Use at 1:1,000 to 1:5,000 dilution. ELISA - Recommended starting diultion of 1:10,000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Background:
The affinity purified anti rabbit IgG reacts with rabbit immunoglobulins and the papain derived Fc and Fab fragments by IEP and gel diffusion techniques. There is no reaction with mouse or human serum. Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum, in Freund's adjuvant. Eggs were collected, the IgY fraction prepared and the anti rabbit IgG immunoaffinity purified off a solid phase immunoabsorbant. Anti Rabbit IgG was further absorbed against mouse and human IgG. The affinity-purified antibody was conjugated with horseradish peroxidase.

Specificity:
Rabbit

Cross Reactivity:
Rabbit

Tested Applications:
ELISA, Immunoelectrophoresis, Western blot. The usefulness of this product in other applications has not been determined.

Host:
Chicken

Clonality:
Polyclonal

Species secondary against:
Rabbit

Conjugation:
Horse Radish Peroxidase

Form:
Liquid

Concentration:
1 mg/ml

Storage Buffer:
0.01M Phosphate Buffered Saline, 0.025% sodium azide

Storage Instruction:
Store at 4 °C.

Affinity Purified Goat anti Human Lambda

admin@catalogchinese.com (Hailiang Wu) — Fri, 29 Aug 2008 23:39:41 -0600

Product Name
Affinity Purified Goat anti Human Lambda

Catalog Number
GTX77552

Product Description:
Affinity Purified Goat anti Human Lambda

Background:
The affinity purified anti lambda reacts with both bound and free lambda chains. It does not react with bound or free kappa chains by IEP and double diffusion analysis.. Goats were immunized with highly purified human lambda chains from normal IgG. Following immunization in Freund's adjuvant the goats were bled, serum collected and the antiserum absorbed with a kappa containing immunoabsorbant. The anti lambda was isolated from an IgG lambda affinity column

Specificity:
Human

Cross Reactivity:
Human

Host:
Goat

Species secondary against:
Human

Form:
Liquid

Storage Buffer:
PBS, pH 7.2, 0.1% sodium azide

Storage Instruction:
4 °C

Affinity Purified Chicken anti-Human IgG h+l

admin@catalogchinese.com (Hailiang Wu) — Fri, 29 Aug 2008 23:49:22 -0600

Product Name
Affinity Purified Chicken anti-Human IgG h+l

Catalog Number
GTX77534

Product Description:
Affinity Purified Chicken anti-Human IgG h+l

Background:
The anti IgG shows a precipitin arc against IgG in serum and against both the Fab and Fc fragments of papain digested IgG using immunoelectrophoresis. Elisa: Recommended starting dilution 1:2000 (coating)1:10,000 (probing) Blotting: 1:5000.. Chickens were immunized with highly purified normal pooled human IgG in Freund's adjuvant and eggs were collected. The IgY fraction was prepared and the antibody immunoaffinity purified

Specificity:
Human

Cross Reactivity:
Human

Host:
Chicken

Species secondary against:
Human

Form:
Liquid

Concentration:
1 mg/ml

Storage Buffer:
PBS, pH 7.2, 0.1% sodium azide

Storage Instruction:
4 °C

Digoxin Antibody Coated ELISA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Sun, 31 Aug 2008 23:54:38 -0600

Suggested use	Competitive Binding ELISA Digoxin
Thermostability	3 years shelf life
Monobind offers 96-well, break-apart plates coated with Digoxin for use in manual or automated systems. These highly versatile plates provide a convenient, fast, and highly efficient framework for any type of immunoassay research work.

Free T4 Antibody Coated CLIA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Sun, 31 Aug 2008 23:59:47 -0600

Monobind offers 96-well, break-apart plates coated with Free T4 for use in manual or automated systems. These highly versatile plates provide a convenient, fast, and highly efficient framework for any type of immunoassay research work.

Free T4 Antibody Coated CLIA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 00:06:56 -0600

Free T4 Antibody Coated ELISA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 00:07:23 -0600

T3 Antibody Coated CLIA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 00:07:48 -0600

Monobind offers 96-well, break-apart plates coated with T3 for use in manual or automated systems. These highly versatile plates provide a convenient, fast, and highly efficient framework for any type of immunoassay research work.

T4 Antibody Coated CLIA Microplate - 96 wells

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 00:08:02 -0600

Monobind offers 96-well, break-apart plates coated with T4 for use in manual or automated systems. These highly versatile plates provide a convenient, fast, and highly efficient framework for any type of immunoassay research work.

anti-HIV-1 gp41 IgG1

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:07:31 -0600

Description	HIV-1 gp41 IgG1 (monoclonal) Produced against a recombinant protein.
Application	ELISA, WB, IP
Recognition	N/A
Clone	1D4
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-HCV core IgG2a

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:09:07 -0600

Description	HCV core IgG2a (monoclonal) Produced against a recombinant protein.
Application	ELISA, IP
Recognition	N/A
Clone	1-E-5
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-HSV (I) gD IgG1

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:15:08 -0600

Description	HSV (I) gD IgG1 (monoclonal) Produced against a HSV (I) viral concentrate
Application	ELISA, WB
Recognition	N/A
Clone	1-I-9
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-HCMV gB IgG1

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:16:30 -0600

Description	HCMV gB IgG1 (monoclonal) Produced against a HCMV infected cells.
Application	ELISA, WB, IP
Recognition	N/A
Clone	1-M-12
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-HBcAg core IgG1

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:18:26 -0600

Description	HBcAg core IgG1 (monoclonal) Produced against recombinant HBcAg core antigen.
Application	ELISA, IHC, FC, IP
Recognition	N/A
Clone	10-E-11
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-Ad1 E11 IgG2a

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:20:40 -0600

Description	Ad1 E11 IgG2a (monoclonal) Produced against purified Ad1 virus
Application	ELISA, WB, IP
Recognition	N/A
Clone	1-M-13
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

anti-Rotavirus p42 IgG2a

admin@catalogchinese.com (Hailiang Wu) — Mon, 01 Sep 2008 21:21:16 -0600

Description	Rotavirus p42 IgG2a (monoclonal) Produced against purified bovine rotavirus virus.
Application	ELISA, WB, IP
Recognition	N/A
Clone	1-O-15
Product size	100 ug/vial
Storage	Long Term: -80C; Short Term: 4C
Shipping	Wet Ice

ChAT (Choline Acetyltransferase) chicken polyclonal anti-peptide antibody

admin@catalogchinese.com (Hailiang Wu) — Fri, 05 Sep 2008 21:32:32 -0600

Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate corresponding to a region of the Choline Acetyltransferase gene product (EC 2.3.1.6) shared between the mouse (Q03059) and human (P28329) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fraction were purified from the yolks. These IgY fractions were then affinity-purified using peptide columns, the concentration of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized.

CNPase (2',3'-cyclic nucleotide phosphodiesterase) chicken polyclonal anti-peptide antibody

admin@catalogchinese.com (Hailiang Wu) — Fri, 05 Sep 2008 21:41:51 -0600

Antibody Concentrations	100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard).
Volume	Regular vials contain 1000 ul; Sampler vials contain 200 ul.
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control	Immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions	1:1000 - 1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. 1:2000-1:5000 for westerns. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized.

Coronin 1a chicken polyclonal anti-peptide antibody

admin@catalogchinese.com (Hailiang Wu) — Fri, 05 Sep 2008 21:42:57 -0600

Antibody Concentrations	100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard).
Volume	Regular vials contain 1000 ul of this antibody mixture; Sampler vials contain 200 ul of this mixture.
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control	The antibody was analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.#F-1005) as the secondary reagent.
Recommended Dilutions	1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate corresponding to a region of the Coronin 1a gene product shared between the mouse (NM_009898) and human (NM_007074) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fraction were purified from the yolks. These IgY fractions were then affinity-purified using peptide columns, the concentration of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized.

Netrin-1 chicken polyclonal anti-peptide antibody

admin@catalogchinese.com (Hailiang Wu) — Fri, 05 Sep 2008 21:44:32 -0600

Antibody Concentrations	100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard)
Volume	Regular vials contain 1000 ul of this antibody mixture; Sampler vials contain 200 ul of this mixture.
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control	This antibody preparation was analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions	1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

MONOCLONAL ANTIBODY DATA SHEET

admin@catalogchinese.com (Hailiang Wu) — Thu, 11 Sep 2008 23:48:07 -0600

SUBCLASS: IgG1

IMMUNOGEN: Ampicillin

SPECIFICITY: NA

CROSS REACTIVITY: NA

CONCENTRATION: 1 - 5 mg/ml

AFFINITY: NA

BUFFER: PBS, pH 7.3

PRESERVATIVE: 0.09% NaN3

STERILITY: 0.2µ filtered

STORAGE:
Short Term: 4 - 8°C
Long Term: -20°C, Avoid repeated freezing and thawing.

INTENDED USE: FOR REMANUFACTURE OR RESEARCH USE ONLY.
PERFORMANCE CHARACTERISTICS MAY DIFFER BY ASSAY SYSTEM.
USERS MUST ESTABLISH PERFORMANCE CHARACTERISTICS FOR

MONOCLONAL ANTIBODY DATA SHEET

admin@catalogchinese.com (Hailiang Wu) — Fri, 12 Sep 2008 00:11:05 -0600

LOT NUMBER: Lot specific

CLONE NUMBER: 48207

SUBCLASS: IgG2a

IMMUNOGEN: Purified R2 from bovine brain

SPECIFICITY: Reacts with 53K band corresponding to R2 in immunoblotting
of human brain extracts. The same immunoreactivity is
observed with R2 from bovine and swine brains.

CROSS REACTIVITY: NA

CONCENTRATION:   Lot specific

AFFINITY: NA

BUFFER: PBS, pH 7.4

PRESERVATIVE: 0.1% NaN3

STERILITY: NA

STORAGE:
Short Term: 4 - 8°C
Long Term: -20°C, Avoid repeated freezing and thawing

MONOCLONAL ANTIBODY DATA SHEET

admin@catalogchinese.com (Hailiang Wu) — Fri, 12 Sep 2008 00:47:39 -0600

SUBCLASS: IgG1k

IMMUNOGEN: Human Corticoliberin conjugated to BSA

SPECIFICITY: Recognizes an epitope of native human/rat CRF,
different from clone 48192

CROSS-REACTIVITY: NA

CONCENTRATION: Lot specific

AFFINITY: Kd = 1.5 x 108
M

BUFFER: PBS, pH 7.4

PRESERVATIVE: 0.1% NaN3

STERILITY: NA

STORAGE:
Short Term: 4 - 8°C
Long Term: -20°C. Avoid repeated freezing and thawing

MONOCLONAL ANTIBODY

admin@catalogchinese.com (Hailiang Wu) — Fri, 12 Sep 2008 00:47:49 -0600

LOT NUMBER: Lot specific

CLONE NUMBER: 48183

SUBCLASS: IgG1

IMMUNOGEN: Human Epidermal Growth Factor (EGF)

SPECIFICITY: Recognizes human EGF with Kd=1.0 x 108
M

CROSS-REACTIVITY: NA

CONCENTRATION:   Lot specific

AFFINITY: NA

BUFFER: PBS, pH 7.4

PRESERVATIVE: 0.1% NaN3

STERILITY: NA

STORAGE:
Short Term: 4 - 8°C
Long Term: -20°C, Avoid repeated freezing and thawing